Journal: World journal of gastroenterology

Article Title: CXCR4/SDF-1 axis is involved in lymph node metastasis of gastric carcinoma.

doi: 10.3748/wjg.v17.i19.2389

Figure Lengend Snippet: Figure 4 Expression of CXC chemokine receptor-4 in gastric carcinoma tissues and stromal cell-derived factor-1 in lymph nodes. A: CXC chemokine recep- tor-4 (CXCR4) protein was detected by immunohistochemistry in primary gastric carcinoma tissues; B: CXCR4 protein was not detected in normal mucous membrane; C: Stromal cell-derived factor-1 (SDF-1) protein was detected by immunohistochemistry in lymph nodes with gastric cancer cell metastasis; D: SDF-1 protein was not detected in normal lymph nodes (400 ×).

Article Snippet: Subsequently, the slides were pretreated with 1% bovine serum albumin in phosphate-buffered saline (PBS) and incubated with antiSDF-1 antibody (Boster; dilution 1:100) and anti-CXCR4 antibody (Boster; dilution 1:50) for 1 h at room temperature.

Techniques: Expressing, Derivative Assay, Immunohistochemistry, Membrane

Journal: World journal of gastroenterology

Article Title: CXCR4/SDF-1 axis is involved in lymph node metastasis of gastric carcinoma.

doi: 10.3748/wjg.v17.i19.2389

Figure Lengend Snippet: Figure 5 mRNA expression of CXC chemokine receptor-4 in gastric can- cer cells, tumors and normal mucous membranes and of stromal cell- derived factor-1 in lymph nodes. A: CXCR

Article Snippet: Subsequently, the slides were pretreated with 1% bovine serum albumin in phosphate-buffered saline (PBS) and incubated with antiSDF-1 antibody (Boster; dilution 1:100) and anti-CXCR4 antibody (Boster; dilution 1:50) for 1 h at room temperature.

Techniques: Expressing, Derivative Assay

Journal: Cell

Article Title: Mobilization-based chemotherapy-free engraftment of gene-edited human hematopoietic stem cells

doi: 10.1016/j.cell.2022.04.039

Figure Lengend Snippet:

Article Snippet: Anti-human CD184 , Miltenyi Biotec , Cat# 130-117-519; RRID:AB_2734059.

Techniques: Blocking Assay, Plasmid Preparation, Recombinant, Electroporation, CRISPR, Adjuvant, Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Cell Culture, Gene Expression

Journal:

Article Title: Bicyclams, Selective Antagonists of the Human Chemokine Receptor CXCR4, Potently Inhibit Feline Immunodeficiency Virus Replication

doi:

Figure Lengend Snippet: Inhibition of binding of the anti-CXCR4 MAb to feline thymocytes in the presence (C) or absence (B) of AMD3100 at 1 μg/ml. In panel A, results obtained with an isotype control MAb are shown. The percentages of fluorescence-positive cells and the mean fluorescence intensity values are indicated in each histogram.

Article Snippet: AMD3100 had no inhibitory effect (at 100 μg/ml) on the binding of feline anti-CD4 MAb (MCA 1347; Serotec, Oxford, United Kingdom) and anti-CD8 MAb (MCA 1350; Serotec) in feline thymocytes. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 4 caption a7 Inhibition of binding of the anti-CXCR4 MAb to feline thymocytes in the presence (C) or absence (B) of AMD3100 at 1 μg/ml.

Techniques: Inhibition, Binding Assay, Fluorescence